Caring for the Tank
TANK CLEANING
This applies mainly to tank maintenance after the fish leave the breeder basket. The most important job after the hatchlings are in place is to keep the tank system clean and the bacteria colonies growing and happy.
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Whenever possible, do your tank work without putting your hands or those of students into the water.
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If you need to put hands in the tank, wash hands in de-chlorinated water—trout are extremely sensitive to chlorine—to remove all contaminants (such as soap and lotion) and dry them thoroughly. Proper hand care when working in the tank will ensure a higher trout survival rate.
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Remove dead and sick-looking fish from the tank immediately. Some fish may start to get lethargic or have problems swimming. Eventually, they simply float around the tank or sink to the bottom, die, and decay. Even one dead fish, if left too long, can spread disease and endanger the whole population
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The gravel should be cleaned twice a week (e.g., Tuesday and Friday). Clean half of the gravel each of these days. During one cleaning, use the siphon to suck up fish waste and dirt from the non-gravel portion of the bottom of the tank and half of the graveled part of the tank. Gravel is cleaned by moving the siphon through and under the gravel, sucking up water and fish waste trapped in and below the gravel. Use one of the 5-gallon buckets to collect the wastewater. Clean the remaining portion of the gravel in the tank during the next semi-weekly cleaning.
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Occasionally, fingerlings can get sucked up along with dirt from the gravel. Just net them and return the runaways to the tank. They may look dispirited or even comatose, but the odds are that they will survive.
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Only remove as much water as needed to clean the gravel and replace that water with water that has been treated with NovAqua Plus water conditioner. (See Appendix E for instructions.) As the fish grow, it may be necessary to increase the frequency of weekly gravel cleanings. Even though about 80% of biological activity takes place in the filter, gravel in the tank serves as part of the tank’s biological filter.
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Weekly remove the slime and dirt that accumulate on the sides of the tank, using a hand mitt, a long handled brush, or some other suitable implement. As the trout increase in size, bi-weekly cleaning of this sort may be required.
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Weekly examine the filter intake and remove tank debris, as well as any dead or trapped fingerlings found there.
B. MAINTAINING CHEMICAL BALANCE IN THE TANK
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Eighty percent or more of the biological activity of the trout tank takes place in the filter. The goal of the tank development process is to “seed” the filter with bacteria that play three different roles:
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decomposition
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nitrification
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denitrification
​By maintaining a healthy water chemistry balance for trout, the need for water changes is minimized.
High ammonia and nitrite levels indicate a lack of adequate biological nitrification. Nitrification is the biological oxidation of ammonia or ammonium to nitrite followed by the oxidation of the nitrite to nitrate. Check KH and add baking soda if required. Add 20 ml of Nite-Out II to the filter to increase ammonia removal. Nitrite and Nitrate are the byproducts of nitrification. Water changes are an additional method of correcting ammonia problems.
Be aware, however, that some short-term spiking of ammonia, nitrite, and nitrate readings is normal. Don’t over-react and increase the size and frequency of water changes unless an ammonia spike is accompanied by signs of fish distress. Almost certainly your fish, through their behavior, will tell you if your water chemistry is seriously out of balance. High ammonia and nitrite levels prevent fish from absorbing oxygen through their gills, at which time the gills darken and may take on a brown color. Fish will be seen at the surface gasping for air or swimming erratically. This is the time to take remedial action by adding 20 ml of Nite-Out II and performing a water change. But if your fish look good, if they’re eating and swimming normally, you might not need to do anything.
The ammonia drives the nitrification process of the Nite-Out II bacteria. Because our tanks typically contain more fish than is recommended for a 55-gallon tank, removal of fish and food waste by vacuuming the tank bottom should be a normal part of your and your students’ routine maintenance.
At the early stages of development, only 2-3 gallon water changes may be necessary. As your fish grow, and food portions increase you may need to change about 5 gallons of tank water at a time. The bacteria in your tank should provide the first line of defense against changes in your tank that affect water chemistry balance. Water changes are secondary to biological activity. Allowing the bacteria to do their job will reduce your need for water changes. The log of daily water testing and the overall health of the trout will also help you determine how much water to change and when to do so.
Note: The ammonia test produces a value that consists of ammonia plus ammonium. The former is un-ionized (NH3) the latter is ionized (NH4+). Ammonia is hazardous to fish and plants; ammonium is not. The test reading is a measure of the sum of both. However, it does not indicate the percent distribution of each component. Therefore, if the test yields an elevated ammonia reading but the fish show no sign of distress, it is very likely ammonium is the larger component of the reading. At lower temperatures (520-540F.) and pH between 7.0 – 7.8 the ammonium value predominates. Unless the fish show signs of distress, there is no need to panic if ammonia readings seem on the high side. At pH readings above 7.8, ammonia toxicity increases.
How temperature and pH affect ammonia
Ammonia varies in toxicity at different pH and temperature of the water. For example, ammonia (NH3) continually changes to ammonium (NH4+) and vice versa, with the relative concentrations of each depending on the water's temperature and pH. At higher temperatures and higher pH, more of the nitrogen is in the toxic ammonia form than at lower pH.
At what point should you get concerned about ammonia levels becoming a threat to your fish given that ammonia is constantly being produced? The answer to this question will depend on the temperature and pH of your tank water, how many fish are in your tank, and how much uneaten fish food remains in the system.
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This chart identifies the level of ammonia you can tolerate in your fish tank before it affects the fish. You will notice that at very warm water temperatures a small amount of ammonia can be toxic to your fish. At the opposite end of the spectrum in very cold water, the opposite is true. Fish can tolerate higher levels of ammonia the cooler the water. This is also true for dissolved oxygen. Cold water can store more dissolved oxygen than the same volume of warm water. The good news is that the water temperatures and pH levels at which our trout are raised tend to reduce the effect of harmful ammonia. If you encounter an ammonia spike that is causing fish mortality you may try lowering the water temperature 2-4 degrees to see if the fish start to recover. Example: 10° C = 50° F. At pH of 7.6, the ammonia test reading would have to exceed 2.8 ppm (interpolate between 3.2 ppm and 2.4 ppm) before it became significant.

Water changes
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Water changes should be performed as needed. The best way to remove water from the tank is by siphoning because this also removes excess waste. In an emergency, an alternative to siphoning is to use a clean gallon jug to scoop water out of the tank. Remember, the jug and the hands of those dipping the jug should be clean and chlorine-free.
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Fill a clean 5-gallon plastic bucket with tap water equal to half the amount of water to be removed from the tank. Add the appropriate amount of NovAqua Plus water conditioner to de-chlorinate (and remove harmful heavy metals from) the water being added. Then add the remaining half of the water being removed. One ml. treats 2 gallons of water. Slowly add the de-chlorinated water to the tank. When done twice a week, this procedure achieves a weekly routine water change that helps keep trout mortality low.
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If the fish appear stressed or start dying in large numbers, it is possible your tank is experiencing an ammonia spike due to a lack of adequate nitrification. Check the ammonia level. If high, assure KH levels are correct, and then add 20 ml Nite-Out II to increase biological ammonia removal. Contact your TIC volunteer for assistance. Correcting the problem may require a large water change, but it is best to proceed with this only after receiving advice.
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When convenient or necessary, water changing may be combined with tank cleaning. As fish grow and feed rates are increased ammonia production is also increased. Since the nitrification process is critical to fish survival always control essential KH levels and add Nite-Out II when ammonia levels increase.
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Always keep two or more 1- or 2-liter bottles of de-chlorinated water in the freezer to maintain the tank water at 52° F in case of a temperature spike caused by a chiller or power failure. The outer surface of these bottles must be cleaned and then rinsed with de-chlorinated water before freezing.
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CHILLER MAINTENANCE
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Once a month, check the chiller intake cooling fins for lint and dust. If necessary, dislodge the dust with a stiff paint brush or tooth brush and use a small hand vacuum to collect the dust. A build-up of dust can cause the compressor to overheat and fail.
CHECK LIST Daily
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Check tank temperature. A temperature increase might indicate a chiller problem.
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Once your fish are feeding, feed the trout (see Chapter 8 for feeding guidelines).
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Remove dead fish or debris from the tank.
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Update data records based on recent water testing and observational reports.
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Ensure that (a) water is flowing from the filter, (b) no fry are caught at the intake points, and (c) the air stone is working properly.
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Check the filter and the air pump hose connections to ensure there are no leaks.
CHECK LIST Two or three times a week
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Test water chemistry (pH, ammonia, nitrites, and nitrates) and record the readings on a Log Sheet (see Tank Inspection Record, Appendix C). Daily testing encourages participation by more students and is optimal from the standpoint of trout health. (In the early stages of the TIC process, especially before your fish have begun to feed, water chemistry should be stable. If that’s the case, you can, if you want, reduce the frequency of your water testing regimen.)
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Clean/siphon gravel as instructed above. Persistently high ammonia or nitrite levels may indicate the need for more frequent or more thorough gravel cleaning.
CHECK LIST Weekly
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Perform the KH test and record the readings in the log. If KH has dropped to 100 or below, use baking soda to raise it. (See Appendix F for instructions on how to use baking soda to correct low KH.)
REPAIR AND REPLACEMENT OF TIC EQUIPMENT
Regardless of how obtained, each school is responsible for the care, maintenance and replacement of its VTTIC equipment.
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Chillers. TradeWind chillers have a 5-year warranty. The invoice for each school’s chiller should be kept in the school’s VTTIC file in order to determine whether the unit is still under warranty in the event of failure. Save the original box in case the unit needs to be returned to the manufacturer. Send a TIC coordinator an email with the serial number of the unit. It is located on the unit’s instruction sheet. The cost of warranty repair is the cost of shipping the unit to the manufacturer (about $75).
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Although the reliability of chillers is high, eventually failures will occur. VTTIC will try to provide a spare unit within 24 hours of a notice of failure if one is available. If the broken unit is no longer under warranty, two options exist. (1) Replace with a new chiller. VTTIC should be notified of this intent. (2) Refurbish the unit. After consulting the manufacturer, VTTIC will recommend whether the unit should be discarded or refurbished. Refurbishing costs vary with the condition of the unit and have ranged between $100-$150 plus shipping. To refurbish a non-warrantied unit, the school pays for shipping both ways. Total repair cost, including shipping both ways, would be about $300. If a school decides to replace its unit, VTTIC may offer to take over and pay for refurbishing the broken chiller to use as a spare. In 2023, a new unit cost $625. Some schools have applied for a grant to cover the cost of replacing their chillers. When a school receives its new/refurbished chiller, any loaned unit should be returned to VTTIC.
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Tanks. Very few failures have been experienced with fish tanks if carefully maintained, handled, and stored. It is difficult to repair a leaking tank. It is best to replace the tank with a new one. Cost is about $130 unless the school can time its purchase to coincide with one of PETCO’s sales. Check with VTTIC to see if any spare tanks are available. Some schools are able to obtain a tank from the PTA/PTO or parents. Grant money is sometimes available to replace equipment. However, if you wait until a failure occurs, it is possible that your grant application would not be acted upon until the next donor funding cycle. Also donors might give higher priority to a grant request from a new VTTIC school than for replacement of existing equipment.


